Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The common DEGs of four gene expression profiles (adj. P value <0.05, |logFC|> 2.0).

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Gene Expression

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: PPI network and hub genes' identification. (a) PPI network was structured by the 54 upregulated DEGs using STRING database. (b) The top 10 hub genes in the PPI network were screened ground on their connectivity degree. The genes such as CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA are represented from red (high degree) to yellow (low degree). (c) Gene Ontology (GO) chord diagram of the top genes in GBM.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Top ten hub genes with higher degree of connectivity.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Binding Assay

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: The AUC analysis of hub genes. AUC analysis of CD44, COL1A1, COL1A2, COL3A1, FN1, IGFBP3, LOX, POSTN, TIMP1, and VEGFA for distinguishing GBM samples from normal tissues.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: OS, DFS, and univariate and multivariate Cox analysis of the hub genes overexpressed in GBM patients. (a) OS of the hub genes overexpressed in GBM sufferers. (b) DFS of the hub genes overexpressed in GBM sufferers. (c) Univariate and multivariate Cox analysis of TIMP1 expression and other clinical pathological factors for OS. HR, hazard ratio.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation between immune infiltrates and TIMP1 expression in GBM. (a) TIMP1 expression was definitely connected with CD4+ T cell, B cell, T cell regulatory (Tregs), neutrophils, cancer-associated fibroblast, macrophage M1, and myeloid dendritic cell. (b–d) Higher infiltration of Tregs, cancer-associated fibroblast, and mast cell correlated with worse prognosis.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Expressing

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Correlation analysis between TIMP1 and related genes and markers of immune cells in TIMER.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques:

Journal: Journal of Oncology

Article Title: Identification of Prognostic Biomarkers of Glioblastoma Based on Multidatabase Integration and Its Correlation with Immune-Infiltration Cells

doi: 10.1155/2022/3909030

Figure Lengend Snippet: Downregulation of TIMP1 inhibits cell proliferation, migration, and invasion in U-87 MG and U-118 MG cells. (a, b) The results of qPCR and WB showed that compared with many glioblastoma cell lines, U-87 MG and U-118 MG cell lines were selected for TIMP1 gene related function test. (c, d) qPCR and WB showed that TIMP1 expression was inhibited in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (e, f) CCK-8 and colony formation assays revealed that the proliferation abilities were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. (g, h) Transwell assay revealed that cell migration and invasion were suppressed in U-87 MG and U-118 MG cells transfected with sh-TIMP1 compared with the NC group. ∗ P < 0.05; ∗∗ P < 0.01; ∗∗∗ P < 0.001.

Article Snippet: After blocking with 5% fat-free milk, incubate with primary antibodies against TIMP1 (A00561-1; Boster, China) and β -actin (Beyotime Biotechnology, China), respectively, using a dilution of 1 : 1000 for each antibody overnight at 4°C.

Techniques: Migration, Expressing, Transfection, CCK-8 Assay, Transwell Assay

Journal: Molecular Medicine Reports

Article Title: Gaseous signalling molecule SO 2 via Hippo-MST pathway to improve myocardial fibrosis of diabetic rats

doi: 10.3892/mmr.2017.7714

Figure Lengend Snippet: SO 2 improves myocardial fibrosis in diabetic rats. (A) Morphological changes in myocardium assessed by Masson staining. Images were acquired at ×400 magnification. Expression levels of (B) MMP9, (C) MMP24 and (D) TIMP1 in each group. Date are expressed as mean ± standard deviation (n=3). *P<0.05 vs. control group; # P<0.05 vs. STZ group. SO 2 , sulfur dioxide; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase; STZ, streptozotocin; HDX, L-Aspartic acid β-hydroxamate.

Article Snippet: The antibodies for matrix metalloproteinase (MMP)9, MMP24, tissue inhibitor of metalloproteinase (TIMP)1 and GAPDH were purchased from Wuhan Boster Biological Technology, Ltd. (Wuhan, China).

Techniques: Staining, Expressing, Standard Deviation, Control